More than 350 PCS and Unite union members at the Equality and Human Rights Commission walked out for an hour on Monday in a bid to save their service.
Offices in Manchester, London, Birmingham, Cardiff and Glasgow were hit.
The commission faces a funding cut of 68 percent from when it was formed in 2007.
Bosses want to more than halve staff to 200 within 12 months, while retaining many consultants and short-term contractors who collectively cost over £6 million a year.
Commission chair Trevor Phillips recently told the Policy Exchange think tank that he always intended to slash staff numbers, “even before the government’s spending review”.
Low paid case workers, outreach advisers and helpline workers who provide advice to the public are facing redundancy or transfer to a private company.
The commission has made no attempt to settle the dispute, and the unions are now stepping up their campaign.
PCS members had already walked out for an hour on two previous occasions.