Train signal workers in Stirling struck for 24 hours on Wednesday of last week.
The strikers, members of the RMT union, want Network Rail to introduce a 12-hour shift system.
Two further 48-hour strikes have been lined up. These will be starting from 1am on 31 May and 1am on 7 June.
A referendum conducted by the RMT found workers favoured the 12-hour system by a margin of 15 to 1.
The strike ballot last month returned a 100 percent vote for action.
RMT union members at Freightliner in Southampton are set to ballot for strikes in a dispute over rosters.
The ballot opens on Wednesday of this week and closes on 30 May. The RMT is recommending a yes vote for action.